Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268

Most Read

Home Articles Most Read
  • Research Paper 2021-09-30

    Screening of Various Fungal Strains from Nuruk and Simultaneous Saccharification and Fermentation by Starch Degradation Activities

    Seong-hwan Yeom, Deok-Ho Kwon, Myoung-Dong Kim, Bong-Hwan Chung, and Suk-Jin Ha

    Abstract : Various fungi were isolated from Korean traditional Nuruk and identified as ten types of fungal strains such as L. corymbifera, L. ramose, S. racemosum, R. oryzae, R. pusillus, M. racemosus, M. circinelloide, A. luchuensis, A. flavus, and A. fumigatus. Among them, four strains (A.fumigatus, A. luchuensis, A. flavus, and R. oryzae) exhibited relatively high glucoamylase activities. When the optimum reaction temperatures were verified for glucoamylase activity, A. fumigatus, A. luchuensis, and A. flavus showed the highest glucoamylase activity at 60°C, and R. oryzae showed the highest glucoamylase activity at 50°C. When simultaneous saccharification and fermentations were performed with yeast, R. oryzae showed the fastest starch degradation rate (1.17 ± 0.10 g/L·h) and the highest ethanol production (17.17 ± 1.46 g/L) for 72 hours.

    Show More  
  • Review Paper 2021-12-31

    Electropolymerized Molecularly Imprinted Polymers (EMIPs)-Based Electrochemical Sensor

    Chae Hwan Cho, Ji-Hong Kim, Jae Hwan Shin, Hyo-Jung Yang, and Jong Pil Park*

    Abstract : Biosensors have been widely studied and developed as a tool in various fields, including pharmaceutical, food safety, environmental and medical applications. Antibodybased immunosensors are considered as gold standard method, however, more advanced biosensor without need of antibody as receptor is needed. Molecularly imprinted polymers (MIPs) are synthetic receptors obtained by polymerization of functional monomers in the presence of the target molecule and can form high specific binding sites to the target molecules depending on shape, size, and functionality. One of the most synthesis of MIPs is electropolymerization that use electrochemical method. This method can easily manipulate the desired morphology and thickness of the polymer films. Various EMIPs (electropolymerized molecular imprinted polymers) based biosensors have been developed for detection of small molecule such as chemicals, drugs, and toxins. In this review, we summarize the synthetic methods and characteristics of EMIPs-based biosensor for various targets.

    Show More  
  • Review Paper 2021-09-30

    Clinical studies of Panax Ginseng and Red Ginseng on Chronic Metabolic diseases: Literature Review

    Soo Hyun Park, Sangwon Chung, Jin-Taek Hwang, and Jae Ho Park

    Abstract : BackgroundThe pharmacological effects of Panax ginseng and red ginseng have been well known for a long time. However, there are no studies that comprehensively examine the effects of P. ginseng and red ginseng on chronic metabolic diseases. Therefore, this study comprehensively reviewed the effects of P. ginseng and red ginseng on chronic metabolic diseases by synthesizing clinical studies that were individually conducted for diabetes, obesity, hypertension, and hyperlipidemia.Methods and ResultsTo evaluate the efficacy of P. ginseng or red ginseng for chronic metabolic diseases, literatures were collected using various databases from December 2020 to January 2021. For the selected studies, the quality of the studies was evaluated using the Risk of Bias tool, and the efficacy on chronic metabolic diseases was reviewed. For analysis, 9 studies using P. ginseng, 20 studies using red ginseng, and one study using P. ginseng and red ginseng were selected. Most of the selected studies were well designed and had low risk of bias. As a result of analyzing the effects on chronic metabolic diseases, it was confirmed that the biomarkers related to diabetes, hypertension, and hyperlipidemia were improved when taking P. ginseng or red ginseng.ConclusionsUntil now, the individual effects of P. ginseng or red ginseng on hyperglycemia, hypertension, and hyperlipidemia have been verified, but in the future, it is necessary to investigate comprehensive effects through clinical studies in high-risk subjects with chronic metabolic diseases.

    Show More  
  • Review Paper 2021-12-31

    Recent Advances of M13 Virus-based Biosensor for Detection of Various Targets

    Hyo-Jeong Yang, Chae Hwan Cho, Ji Hong Kim, Jae Hwan Shin, and Jong Pil Park*

    Abstract : A biosensor is an analytic microsystem in which a biological component is coupled with a transducer to convert biological signal into an electrical out. Utilization of biosensors has been considered the importance in the field of drug discovery, biomedicine, food safety, biodefense with security, and environmental monitoring. Recently, interest in biosensor is still increasing, and it needs to develop more advanced biosensor that capable of any of targets with high sensitivity and selectivity. Therefore, it is necessary to discover new bioreceptors with high sensitivity and selectivity. Bacteriophage, especially M13 bacteriophages, are emerging as new biological receptors. The peptide-displayed M13 bacteriophage not only could specifically and selectively bind to desired target molecules but also easily manipulate genetical and/or chemical modification in biosensor applications. Therefore, we summarize recent advances and the possibilities of biosensor development utilizing M13 bacteriophages.

    Show More  
  • Research Paper 2021-12-31

    Comparative Analysis of Biological Activity between Domestic Cudrania Tricuspidata Fruit Extract and Fermented Fruit Extract Using Lacto-Bacillus Plantarum

    Moon-Hee Choi, Seung-Hwa Yang, Da-Song Kim, and Hyun-Jae Shin

    Abstract : Cudrania tricuspidata belongs to the Morus family. The fruits and leaves are used for food, and the barks and roots are used medicinally and as raw materials for paper. Heretofore, studies on C. tricuspidata leaves have been actively conducted, but studies on fruits are insufficient. The recent trend in health food is bioconversion and enhancement of active compounds through fermentation. In addition, consumption of natural health food is increasing. Therefore, in this study, the biological activities of C. tricuspidata fruit extracts were measured to use it as a food material. The fruit extracts were fermented with Lactobacillus plantarum, and it was extracted with hot water. Changes in antioxidant activities and polyphenol contents were compared between C. tricuspidata fruits extracts and fermented C. tricuspidata fruits extracts. It was confirmed that the antioxidant activity of the C. tricuspidata fruit fermented product was higher than that of the C. tricuspidata fruit extract. The DPPH radical scavenging activity IC50 of each extract was 330.89 ± 11.65 μg/mL and 87.28 ± 4.20 μg/mL, respectively, and the ABTS IC50 was 335.09 ± 15.71 μg/mL, and 265.29 ± 4.68 μg/mL. The new beverage manufacturing process was developed using the fermented C. tricuspidata fruits extracts, and sensory evaluation of the beverage was conducted. Based on the results of this study, it is necessary to develop foods with sensory palatability and maximizing the active compounds and biological activities of C. tricuspidata fruit.

    Show More  
  • Research Paper 2021-12-31

    Apoptotic Effects from Hexane fractions of Lysimachiae foenum-graeci Herba through Regulating Akt/Mdm2 Signaling Pathways in HCT116 Colon Cancer Cells

    Ji Won Seo, Hyo Jae Lee, Chang Yeol Lee, Sang Yong Kim, and Young Min Kim

    Abstract : Lysimachiae foenum-graeci Herba is a shade-dried whole plants of the Lysimachia foenum-graecum Hance and is well known to have anti-oxidant, anti-obesity and antibacterial effects. Accordingly, in this study, we assessed the apoptotic effects of hexane fractions from Lysimachiae foenum-graecum (LFH) on HCT116 Human colon cancer cells, and to confirm whether this effects are due to the regulation of Akt/Mdm2 signal pathways. As a result of MTT assay, LFH showed cell proliferation inhibitory and cytotoxic effects of HCT116 cells, and confirmed through Annexin V & Dead Cell assay to the staining rate of phosphatidylserine (PS) expressed by apoptosis. In addition, it was confirmed through Hoechst 33342 assay that the fluorescence caused by apoptosis progression and apoptosis bodies were caused by apoptosis induction of HCT116 cells by LFH. And it was confirmed through Western blotting that treatment of LFH induced inhibition of p-Akt, p-Mdm2, Bcl-2, and Pro-caspase 3 and activation of p-AMPK, p53, Bak, Bax, and Cleaved-caspase 3. Furthermore, it was confirmed that the apoptosis induction effect by LFH was caused by Akt/Mdm2 pathway regulation through Akt inhibitor (LY294002) and Mdm2 inhibitor (Nutlin-3) treatment. In conclusion, it was confirmed that LFH induces apoptosis through Akt/Mdm2 signaling pathway in HCT116 Human colon cancer cells.

    Show More  
  • Research Paper 2021-12-31

    Developments of GABA Process for the Mass Production

    Eui Jin Kim, Hyun-Jae Shin, and Jung-Heon Lee*

    Abstract : Gamma-amino butyric acid (GABA) is a component of pharmaceuticals and functional foods. GABA can be biosynthesis through the decarboxylation of glutamate by glutamate decarboxylase (GAD) and this conversion process consume hydrogen ion cause change pH condition. Mass production of GABA process overcome changing pH, supplied HCl each 3 hr when 3 times for prevent pH shift and using 1 M HCl for the inhibition to destroying of GAD structure and added PLP for increasing to GAD activity developed process of mass production that 1M MSG convert to 0.99 M GABA on 15 hour. Consider to GAD activity and produced GABA purity, this process is suitable for mass production process of GABA.

    Show More  
  • Research Paper 2021-12-31

  • Research Paper 2021-12-31

    Over-expression and Host-specific Algicidal Effect of Virus-like Particles from Capsid Gene of HcRNAV34 Virus Infecting against a Harmful alga, Heterocapsa circularisquama

    Sun Il Seo, Jae Won Ryu, and Si Wouk Kim

    Abstract : Harmful algal blooms (HABs) is a rapid increase or accumulation in the population of harmful microalgae in freshwater and marine aquatic system, and can harm the health of the environment, plants or animals. Conventional chemical and physical techniques to mitigate HABs such as chlorination, ozonation, ultrasonic treatment, and clay-based flocculation are limited by the low efficiency and risk of destroying the aquatic ecosystems. Therefore, host-specific virus-like particles (VLPs) as an alternative biological method for the efficient control of harmful microalgae that can be combined with chemically synthesized algicidal compound has been successfully performed in this lab. This system was efficient for algicidal activity but also involved several significant disadvantages such as difficult purification and less production rate. To overcome these issues, we attempted to develop a low-cost, constitutive overexpression, and easy purification system of viral capsid protein to obtain large amounts VLPs. The capsid protein of HcRNAV34 as a singlestranded RNA virus that infects the toxic dinoflagellate, Heterocapsa circularisquama, was successfully expressed in E. coil without inducer. The maximal condition for production of HcRNAV34 capsid protein in the E. coil BL21(DE3) was optimized, and then finally 3.25 g/ℓ (wet weight) cell mass was obtained from 30 L reactor containing M9 medium at 37oC for 20 h. The expressed insoluble capsid protein was partially purified by low speed centrifugation and labelled with green fluorescent FITC. The resulting protein was visualized on the cell surface of H. circularisquama 9433 and 92-1, the hosts of native HcRNAV34 virus. In addition, HcRNAV34 VLPs absorbed with TD49 as algicidal substance showed a high algicidal effect against H. circularisquama 9433 and 92-1 in a host-specific manner. This host target-specific VLPs can be used for the management of HABs in aquatic ecosystems.

    Show More  
  • Research Paper 2021-09-30

    Skin Whitening and Anti-wrinkle Effects of Pleurotus nebrodensis Extracts

    In Hae Kim and Jae Hwa Lee

    Abstract : Pleurotus nebrodensis have been used as a traditional remedy and a food source. Our study focuses on figuring out the possibility of Pleurotus nebrodensis as cosmetic materials. We examined anti-oxidant, anti-wrinkle, anti-inflammation and whitening effects. The SOD-like activity was 69.7 ± 2.31 at 125 μg/mL, compared to the standard sample L-ascorbic acid 1 mM 34.7 ± 0.79. It was 11.5 ± 0.27 mg/g Naringin base and 64 ± 1.45 GAE (Gallic acid) base as a measurement result of total flavonoid and total polyphenol contents. GME showed 24.5% (L-tyrosine), 16.6% (L-DOPA) of tyrosinase inhibition assay, respectively. In elastase inhibition assay showed 36.2% of inhibition at a concentrations 200 μg/mL, tyrosinase and elastase activation were found to inhibited dose-dependent. As a result of measuring the collagen precursor pro-collagen type 1, the amount of collagen synthesis was 434 ng/mL at a concentration of 500 μg/mL. The higher the concentration of GME the greater the wrinkle reduction and whitening effects. Anti-inflammatory activites measured nitric oxide (NO) and cell viability in LPS-induced macrophage RAW264.7 cell, it was found to inhibit NO production in a concentration-dependent manner. These results suggest methanol extracted Pleurotus nebrodensis has excellent activities in the anti-wrinkle, anti-inflammation and whitening. Therefore, Giant mushroom Pleurotus nebrodensis may have value as the potential cosmetic formulations.

    Show More  

Current Issue

March, 2022 Volume 37, No.1

Most Read

Korean Society for Biotechnology and Bioengineering Journal
pISSN 1225-7117 eISSN 2288-8268