pISSN 1225-7117 eISSN 2288-8268

Keratinase expression in engineered host

Expression host Gene (origin) Keratinase type Substrates Optimal conditions Enzyme activity (U/mL) Specific activity (U/mg protein) References
pH Temp (°C) Time (days)
Bacteria Bacillus megaterium ATCC 14945 Bacillus licheniformis ker - Keratin azure - - 0.75 166.21 33.25 [68]
Bacillus subtilis WB600 Bacillus licheniformis ker - Keratin powder 10.5 40 0.75 323 - [69]
Escherichia coli BL21 Bacillus licheniformis kerA - Keratin azure 7.5 50 - 26.8 9.8 [70]
Escherichia coli BL21 Thermoactinomyces sp. kerT1 Endo1) Soluble keratin 8.5 65 0.5 - 20.3 [71]
Escherichia coli HB101 Bacillus licheniformis ker BL Endo Keratin azure 9-10.0 60 1.5 11.25 75 [72]
Yeast Pichia pastoris GS115 Aspergillus oryzae alp - Casein 9.0 30-40 5 47.54 40.94 [73]
Pichia pastoris SMD1168H Pseudomonas aeruginosa apr - Azokeratin 8.0 60 3 1030 1800 [74]
Pichia pastoris X33 Bacillus licheniformis kerA Endo Azokeratin 8.5-9.5 55-60 6 285 - [75]

1)Endo-keratinolytic protease (S8 family).

Korean Society for Biotechnology and Bioengineering Journal 2022;37:1~10 https://doi.org/10.7841/ksbbj.2022.37.1.1
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