pISSN 1225-7117 eISSN 2288-8268

Primers designed for Alu PCR

Primer Name Target locus Usage Sequence (5` → 3`)
COX4 1st FP COX4 First PCR GGA GTG TTG TGA AGA GTG AAG ACT
EcoRI/COX4 2nd FP Second PCR CCG GAA TTC GTC TTC CTG CTT ATA TGG ATC GGC
CMV Promoter 1st RP Transgene First PCR ATA GAC CTC CCA CCG TAC AC
bGH pA Tail 1st FP TGT GCC TTC TAG TTG CCA GC
EcoRI/CMV Promoter 2nd RP Second PCR CCG GAA TTC TCA ATG GGG CGG AGT TGT TAC
EcoRI/bGH pA Tail 2nd FP CCG GAA TTC CAT CTG TTG TTT GCC CCT CC
Tailed clone 49c FP SINEs First PCR ATC GAG TCG UCG GAT CGG TUG CTG GAG AGA TGG TTC AGA GGT
Tailed clone 49c RP ATC GAG TCG UCG GAT CGG TUC CAG AAG AGG GCA CCA GAT CT
Tailed mouse B1 FP ATC GAG TCG UCG GAT CGG TUT GGT GGC GCA CAC CTT TAA TC
Tailed mouse B1 RP ATC GAG TCG UCG GAT CGG TUT CTT TGT GTA GCC TTG GCT GT
Tailed G-repeat FP ATC GAG TCG UCG GAT CGG TUC TCC TAT GCT CCC CAC CTA C
Tailed G-repeat RP ATC GAG TCG UCG GAT CGG TUC CTT GGA GCC AGT GTT TAT CCC
XhoI/Tail Customized tail Second PCR GTT CCT CGA GAT CGA GTC GTC GGA TCG GTT

The whole primers for second PCR have restriction enzyme digestion sites to apply Alu PCR in case of DNA cloning without TA cloning steps.

FP : forward primer, RP : reverse primer

Korean Society for Biotechnology and Bioengineering Journal 2022;37:17~24 https://doi.org/10.7841/ksbbj.2022.37.1.17
© Korean Society for Biotechnology and Bioengineering