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Fig. 3. Alu PCR applied to an endogenous gene. (a) Cytochrome c oxidase subunit 4 (cox4) is 135 bp away from clone 49c. The product size between primers on coding sequence 2 and clone 49c was expected to be 2.1 kb. (b) Results of Alu PCR between cox4 and clone 49c. Thirty cycles of normal PCR were performed using primer pairs for the first PCR step of Alu PCR. CHO-K1 and GSR were used to validate experiment. 2.1 kb of results were identified in only Alu PCR result.
Korean Society for Biotechnology and Bioengineering Journal 2022;37:17~24 https://doi.org/10.7841/ksbbj.2022.37.1.17
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