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Fig. 4. Alu PCR results applying to recombinant cell lines. (a) The FP of each SINEs and CMV promoter of each SINEs were used to detect integration site on genome of two cell lines (Alb-EPO and CTLA4Ig). Two remarkable results (lane 1 and lane 8) were identified. Unlike two results, no results identified on GSR (data now shown). (b) Transgene integration site of CTLA4Ig analyzed using the result of lane 8. Transgene was inserted in intron between exon 23 and 24 of plekstrin homology domain containing A5 (plekha5) gene of chromosome 8. Black one-head arrows indicate primers for Alu PCR. (c) The FP and RP of each SINEs and bGH poly A tail were used to detect integration site of GSR cell line. Lane 17 was considered the most likely result of Alu PCR. No results identified on the other two types of cell lines (data not shown). (d) Integration site of GSR. Gene was inserted in intron between exon 5 and 6 of tetratricopeptide repeat protein 7a (Ttc7a) gene of chromosome 5. Black one-head arrows indicate primers for Alu PCR.
Korean Society for Biotechnology and Bioengineering Journal 2022;37:17~24 https://doi.org/10.7841/ksbbj.2022.37.1.17
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