pISSN 1225-7117
eISSN 2288-8268
CRISPR/Cas-based POCT
| Cas type | System name | Target | Amplification1 | Time | LOD (copies/μL) | Sensitivity/Specificity (%) | Readout | Instrument | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| Cas13 | BiosensorX | COVID-19, Antimicrobial resistance detection (ß-lactam antibiotic) | None (transcription x) | ~30 min | 2,000 for E gene 7,520 for RdRP 50 for combined | -/- | Electrochemi cal assay (Potentiostat) | Multiplex microfluidic biosensor (BiosensorX) +NFC potentiostat | [65] |
| Cas12 | Instrument-free | COVID-19 | RT-RPA | - | 100 | 94.1/100 | Lateral flow assay in microfluidic system | - | [66] |
| Cas13, Cas12 | ECS-CRISPR (Easy to operate, Contamination - free, and Stable CRISPR) | COVID-19, African swine fever virus (ASFV) | RT-RPA | ~25 min | 3 | -/- | Fluorescence readout | Biosensor | [67] |
1RT-RPA, Reverse transcriptional- Recombinant Polymerase Amplification