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Fig. 3. Systems/synthetic biology-based approaches to overcome sequence and functional spaces of the three essential parts in expression vectors. Limited resources in obtaining replication origins and promoters can be resolved solely or combinatorially by accessing sequence databases and using trap system from metagenomes. As alternative markers for the clonal selection, antibiotics-free selection systems such as auxotrophic marker or hok/sok locus are intentionally equipped in the host and function without any interference with a molecular machine of central dogma.
Korean Society for Biotechnology and Bioengineering Journal 2023;38:100~111 https://doi.org/10.7841/ksbbj.2023.38.2.100
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