pISSN 1225-7117
eISSN 2288-8268
HPLC operation conditions for determination of madecassoside
| HPLC Condition | |||
|---|---|---|---|
| Instrument | e2695 Waters, USA | ||
| Column | Kromasil C18 column, 5 μm, 4.6 × 250 mm (AkzoNobel, Netherlands) | ||
| Mobile phase | Time (min) | Composition | |
| 0.01% Phosphate buffer (%) | Acetonitrile (%) | ||
| 5 | 80 | 20 | |
| 10 | 60 | 40 | |
| 12 | 40 | 60 | |
| 14 | 20 | 80 | |
| 20 | 80 | 20 | |
| Detector wavelength | UV 202 nm | ||
| Injection volume | 10 μL | ||
| Flow rate | 1 mL/min | ||
| Column temperature | 40°C | ||
| Run time | 20 min | ||