Fig. 1. Cytotoxic and anti-proliferative effects of CME in HT-29 colon cancer cells. (a) Cells were treated with variable concentrations of CME (10~100 μg/mL) for 12 h, 24 h and 48 h. Cell viability was measured by MTT assay. The statistical analysis of the data was carried out by use of independent sample t-test. *,#p < 0.05, **,##p < 0.01 and ***,###,+++p < 0.001 vs. con (each experiment, n = 3). (b) LDH release was measured by LDH release assay. The statistical analysis of the data was carried out by use of a one-way ANOVA with Duncan multiple range test (p < 0.05). (c) Anti-migration effects of CME in HT-29 colon cancer cells. Cell were treated with variable concentrations of CME (20~100 μg/mL) for 24 h. Scratch wound healing assay was performed to examine the anti-migration effects of CME. (d) The cell morphology change after treatment of CME was observed by invert microscopes (x200 magnification, Axiovert 100, Zeiss, Germany). N.S., not significant; CME, Cnidium monnieri (L.) Cusson extract; con, control.
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