Fig. 2. Apoptotic and cell cycle arrest effects of CME in HT-29 colon cancer cells. Cell were treated with variable concentrations of CME (40~80 μg/mL) for 24 h. (a) Apoptotic effects of CME were evaluated by Annexin V-PI double staining. (b) The graph of apoptosis rate. (c) Apoptotic bodies were measured by Hoechst 33342 staining. The chromatin was observed using fluorescence microscope (Axioskop 50, Carl Zeiss, Thornwood, NY, USA). Origin magnification x200. (d) CME occur cell cycle arrest at G1 phase. Cell cycle arrest effects were measured by flow cytometric. (e) The graph of cell cycle phase. CME, Cnidium monnieri (L.) Cusson extract.
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