pISSN 1225-7117
eISSN 2288-8268
Sequences of primers used for site-directed mutagenesis. The nucleotide changes are underlined
| Target site | Sequence of Primers |
|---|---|
| M178T | Forward 5’-CGCAAAATTCACACGCTGG-3’ |
| Reverse 5’-TTGCTCCATACCACCGAC-3' | |
| F248L | Forward 5’-CCAAGTATTTACTGGAATACCCAC-3’ |
| Reverse 5’-CGATTTCCATGATCGGGTTAC-3’ | |
| K256E | Forward 5’-CCCGGAGAAATTTGGCGG-3’ |
| Reverse 5’-CGCTCAATCGTCGGTGGGTATTC-3’ |