Fig. 1. Splitting position in chromosome II (825 kb) of FY834/pRS-NGH strain (A). The chromosome II was split into 305 kb and 524 kb-YAC by 1st splitting and YKY179 strain (R-split) was constructed. Subsequently, the 305 kb YAC was successfully split into 270 kb-YAC and 38 kb mini-YAC by 2nd Splitting and YKY180 strain (RL-split) was constructed (B). The SCO2 and MNN2 genes were used as target gene for each splitting.
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