Fig. 2. Confirmation of the split-YACs by Puled field gel electrophoresis (PFGE) and Southern hybridization (A). AGAH71 and CEN4 genes were used as probes. The 38 kb-mini YAC contains NABH558, AGAG1, AGAH71, CEN4, and CgLEU2 genes as foreign genes. Lane 1 FY834/pRS-NGH strain (Host strain), lane 2; YKY179 strain (R-split), lane 3; YKY180 strain (RL-split). Comparison of agarase activity in host strain and transformants containing split-YACs (B). Each strain was grown on YPDG medium and then stained using iodine solution. 1; S. cerevisiae FY834, 2; FY834/pRS-NGH, 3; YKY181 strain (L-split), 4; YKY179 strain (R-split), 5; YKY180 strain (RL-split)
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