pISSN 1225-7117 eISSN 2288-8268

Comparison of cell growth and agarase activity in yeast transformants expressing various agarases

Transformants Dry cell weight (g/L) Agarase activity (unit/mL) Specific activity (unit/g-DCW)
FY834/pRS-H 8.67 0.32 36.9
FY834/pRS-GH 7.89 0.48 60.8
FY834/pRS-NGH 8.01 0.55 68.7
YKY179 (R-split) 6.75 0.43 63.7
YKY180 (RL-split) 6.58 0.45 68.4

These strains were cultivated in 50 mL YPDG medium for 72 hrs.

One unit of agarase activity was defined as the amount of enzyme that produced 1 μmole of galactose per minute under the assay condition.

Korean Society for Biotechnology and Bioengineering Journal 2024;39:136~144 https://doi.org/10.7841/ksbbj.2024.39.4.136
© Korean Society for Biotechnology and Bioengineering