Fig. 4. TLC chromatograms of hydrolysis products of agarase on agarose. The enzymatic reaction of agarase was carried out at 40°C in TE buffer containing 0.5% agarose as a substrate. Lane 1; neoagarooligosaccharide (NAO), lane 2; neoagarotetrose (NA4), lane 3; neoagarobiose (NA2), lane 4; galactose (Gal), lane 5; anhydrogalactose (AHG), lane 6~9; products hydrolyzed by agarase for 4,8, 12 and 24 h, respectively.
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