Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

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Korean Society for Biotechnology and Bioengineering Journal 2009; 24(5): 469-476

Published online October 31, 2009

Copyright © Korean Society for Biotechnology and Bioengineering.

염주괴불주머니 (Corydalis heterocarpa) 의 항산화 활성

Antioxidant Activity of the Salt Marsh Plant Corydalis heterocarpa

김유아(한국해양대학교 해양환경.생명과학부), 이정임(한국해양대학교 해양환경.생명과학부), 이진혁(한국해양대학교 해양환경.생명과학부), 공창숙(부경대학교 해양바이오프로세스연구단), 남택정(부경대학교 식품생명공학부), 서영완(한국해양대학교 해양환경.생명과학부)

Abstract

The antioxidant activities of two crude extracts ($CH_2Cl_2$ and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions) from Corydalis heterocarpa were determined by evaluating authentic $ONOO^-$ and $ONOO^-$ generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic $ONOO^-$ increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions, while those on $ONOO^-$ generated from SIN-1 increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at $10\;{\mu}M$. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.

Keywords: Corydalis heterocarpa, cnidimoside A, nitric oxide, reactive oxygen species, peroxynitrite