Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268

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Korean Society for Biotechnology and Bioengineering Journal 2011; 26(3): 223-228

Published online June 30, 2011

Copyright © Korean Society for Biotechnology and Bioengineering.

Enterobacter aerogenes를 이용한 crude glycerol 기반의 바이오에탄올 생산

Bioethanol Production Based on Crude Glycerol Using Enterobacter aerogenes

정홍섭(광운대학교 화학공학과), 성필제(광운대학교 화학공학과), 고아라(광운대학교 화학공학과), 이상준(고려대학교 화공생명공학과), 김승욱(고려대학교 화공생명공학과), 한성옥(고려대학교 생명과학대학 생명과학부), 조재훈(한국생산기술연구원 그린공정연구부), 조대행(광운대학교 화학공학과), 김용환(광운대학교 화학공학과), 박철환(광운대학교 화학공학과)

Abstract

The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were $1.32\;OD_{600}$ and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process maybe useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.

Keywords: Enterobacter aerogenes, ethanol, glycerol, crude glycerol