Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268

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Korean Society for Biotechnology and Bioengineering Journal 2011; 26(3): 248-254

Published online June 30, 2011

Copyright © Korean Society for Biotechnology and Bioengineering.

염증세포주를 이용한 생체재료 용출물의 체외 생체적합성 평가

In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines

신연호(중앙대학교 병리학교실), 송계용(중앙대학교 병리학교실), 서민지(동국대학교 의생명공학과), 김성민(동국대학교 의생명공학과), 박정극(동국대학교 의생명공학과), 김동섭(한국식품의약품안전청), 박기정(한국식품의약품안전청), 허찬희(한국식품의약품안전청), 차지훈(한국식품의약품안전청), 서영권(동국대학교 생명과학연구원)

Abstract

Various biometerials have been researched and have been developed for treatment of some disease through transplantation to body. They have been evaluated by in vitro cytotoxicity test using some skin-derived cell lines for prediction of their biocompatibility in vivo. However, the results of experiments using mesenchymal or epithelial cells could not be considered in vivo immune reaction. In this study, we evaluated the biomaterial-elution (elute from high density polyethylene film) using some cell lines (L929, Jurkat, U937) in vitro, and then that results were compared with in vivo results from guinea pig sensitization test. In sensitization test, saline and elution of syringe could not induce erythema, but only DNCB (hypersensitive chemical) induce erythema at guinea pig sensitization test. In cell experiment, the cytotoxicity results of inflammatory cells (Jurkat; T lymphocyte, U937; monocyte) was no difference with L929 (fibroblast) in the overall trend. However, inflammatory cell lines were only secreted inflammatory cytokine (TNF-${\alpha}$, INF-${\gamma}$) in some materials (biomateriallution, FAC, DNCB). And the biomaterial-elution did not have toxicity to the cells, but it induced the inflammatory cytokines in inflammatory cell lines only. So, we were predicted inflammatory reaction through the cytokine resultes of inflammatory cell lines, and it was more correlated with in vivo results than cytotoxicity test. Therefore, we suggested that the inflammatory cytokine assay using inflammatory cell lines are more effective method in vitro for evaluation of biocompatibility of biomaterials or chemicals.

Keywords: inflammation, biocompatibility, inflammatory cell line, fibroblast, cytokine