Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

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Korean Society for Biotechnology and Bioengineering Journal 2012; 27(2): 97-102

Published online April 30, 2012

Copyright © Korean Society for Biotechnology and Bioengineering.

두 종 미세 녹조류의 연속배양을 통한 바이오매스 생산성 비교

Comparison of Biomass Productivity of Two Green Microalgae through Continuous Cultivation

김근호(조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀), 이영미(조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀), 김덕진(한국환경공단 상하수도지원처 물산업진흥팀), 정상화(조선대학교 기계공학과), 김시욱(조선대학교 환경공학과, 조선대학교 BK21 바이오수소 생산팀)

Abstract

In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole $CO_2$ source and other culture conditions as follows: light intensity, temperature, pH, $NH_4Cl$ and $K_2HPO_4$ were 100 ${\mu}E/m^2/s$, $27^{\circ}C$, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, $KNO_3$ and $K_2HPO_4$were 80 ${\mu}E/m^2/s$, $27^{\circ}C$, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% $CO_2$ supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% $CO_2$ concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.

Keywords: green microalga, Chlorella, Dunaliella salina, photobioreactor, biomass productivity