Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268


Home All Articles View

Korean Society for Biotechnology and Bioengineering Journal 2012; 27(3): 137-144

Published online June 30, 2012

Copyright © Korean Society for Biotechnology and Bioengineering.

고속 압출 전처리 공정을 이용한 Chlorella sp. 당화 및 바이오에탄올 생산

Saccharification and Ethanol Production from Chlorella sp. Through High Speed Extrusion Pretreatment

이춘근(강원대학교 의생명과학대학 의생명소재공학과), 최운용(강원대학교 의생명과학대학 의생명소재공학과), 서용창(강원대학교 의생명과학대학 의생명소재공학과), 송치호(강원대학교 의생명과학대학 의생명소재공학과), 안주희(강원대학교 의생명과학대학 의생명소재공학과), 정경환(한국교통대학교 보건생명대학 생명공학과), 이상은(한국교통대학교 보건생명대학 생명공학과), 강도형(한국해양과학기술원), 이현용(서원대학교 식품과학부 차학과)


Among various pretreatment processes for bioethanol production, extrusion pretreatment, one of cheap and simple process was investigated to efficiently produce fermentable sugars from micro alga, Chlorella sp. The biomass was pretreated in a single screw extruder at five different barrel temperatures of 45, 50, 55, 60 and $65^{\circ}C$, respectively with five screw rotation speed of 10, 50, 100, 150 and 200 rpm. The pretreated biomass was reacted with two different hydrolyzing enzymes of cellulase and amyloglucosidase since the biomass contained different types of carbohydrates, compared to cellulose of agricultural by-products such wheat and corn stovers, etc. In general, higher glucose conversion yield was obtained as 13.24 (%, w/w) at $55^{\circ}C$ of barrel temperature and 100 rpm of screw speed conditions. In treating 5 FPU/glucan of cellulase and 150 Unit/mL of amyloglucosidase, ca. 64% of cellulose and 40% of polysaccharides in the micro alga were converted into glucose, which was higher yields than those from other reported data without applying an extrusion process. 84% of the fermentable sugars obtained from the hyrolyzing processes were fermented into ethanol in considering 50% of theoretical maximum fermentation yield of the yeast. These results implied that high speed extrusion could be suitable as a pretreatment process for the production of bioethanol from Chlorella sp.

Keywords: Extruder, Pretreatment, Bioethanol, Microalgae, Enzyme treatment