Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268


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Korean Society for Biotechnology and Bioengineering Journal 2013; 28(1): 13-17

Published online February 28, 2013

Copyright © Korean Society for Biotechnology and Bioengineering.

딱지꽃 (Potentilla chinensis) 추출물의 항염증 효과

Anti-Inflammatory Effect of Chloroform Extract from Potentilla chinensis

강창호(인하대학교 생물공학과), 한상현(인하대학교 생물공학과), 소재성(인하대학교 생물공학과)


In this study, we investigated the anti-inflammation effect of Potentilla chinensis (PC) on Raw264.7 macrophage cells. Ethanol extract of PC decreased the production of nitric oxide (NO) in LPS-stimulated RAW264.7 cells. Ethanol extract was fractioned by n-hexane, chloroform, ethyl acetate, n-butanol, water and each fraction was tested for inhibitory effects on inflammation. Among the sequential solvent fractions, PC chloroform extracts (50, 100, 300, and 500 ${\mu}g/mL$) significantly suppressed LPS-stimulated production of NO. During the entire experimental period, 200 and 300 ${\mu}g/mL$ of PC chloroform extracts had no cytotoxicity. LPS-induced NO and prostaglandin $E_2$ ($PGE_2$) production were inhibited by PC chloroform extracts up to 50% and 90% of these productions, respectively. PC chloroform extracts reduced the expression of iNOS and COX-2 gene. These results suggest that PC chloroform extracts exhibit strong effects of anti-inflammation and can be a potential candidate in the treatment of acute and chronic inflammatory diseases.

Keywords: Potentilla chinensis, Nitric oxide, Prostaglandin $E_2$, iNOS, COX-2