Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268

Review Paper 31 December 2023

Recent Advances in Research on Nanotechnology-based Drug Delivery System

Kyong Min Kwak, Jeong Un Kim, and Moon Il Kim Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 203-211
The term “drug delivery system (DDS)” refers to a group of technologies for efficiently managing the distribution of pharmacologically active medicines to target cells and tissues in vivo. The use of appropriate DDS can enhance the activity, stability, and permeability of pharmaceuticals, as well as their side effects can be reduced by treating only the targeted location and keeping the drug levels below the dangerous threshold in physiological fluids. In particular, materials derive...
Key Words: drug delivery system, nanotechnology, lipid nanoparticle, exosome, microneedle, nanoneedle

Research Paper 31 December 2023

Production of Deinoxanthin Using Deinococcus radiodurans R1

Young Woo Shin, Sun-Wook Jeong, Younghun Cho et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 212-216
Xanthophyll carotenoids are pigments composed of terpene structures found in various organisms. They have attracted significant attention in the food, nutrition, pharmaceutical, and cosmetic industries due to their remarkable anti-bacterial, anti-inflammatory, and antioxidant activities. Among these, Deinoxanthin, produced by microorganisms of the Deinococcus genus, is known for its superior radical scavenging activity compared to well-known carotenoids like lutein, beta-carotene, and zeaxanthin...
Key Words: deinoxanthin, carotenoids, Deinococcus, extremophile, crude glycerol, production

Research Paper 31 December 2023

Effect of Different Light Sources and Intensities on the Growth of Astaxanthin-producing Microalgae Haematococcus lacustris (Chlorophyceae)

Tae Yeon Yin, Eun Young Yoon, and Tae Hoon Kim Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 217-224
The green microalga Haematococcus lacustris is a source of astaxanthin, a natural pigment additive with commercial value in functional food, cosmetic, and pharmaceutical industries. Although conventional light sources are being increasingly replaced with light-emitting diodes (LEDs), their applications in microalgal biotechnology remain limited. In this study, we investigated the effects of monochromatic light types, light intensities, and different ratios of mixed light on the growth of two H. ...
Key Words: biomass productivity, Haematococcus lacustris, LED wavelength, light intensity, light source

Research Paper 31 December 2023

Thermodynamic Properties in the Process of Thawing for Cryopreservation of Algae

Min Yong Kim, Young Woo Shin, Young Bok Lee et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 225-235
To achieve a high viability by minimizing thawing damage, ice crystallization must be reduced in the process of thawing. Thermophysical properties of suspensions of Porphyra seriata, Undaria pinnatifida, and Sargassum fulvellum were measured to determine the optimal thawing conditions in the thawing process for species conservation of sea algae. The range of final thawing temperature was –2.49 ~ –0.50°C at thawing speed 1.25°C/min and 2~3°C at 1.5~2.0°C/min, and thawi...
Key Words: cryopreservation, viability, thermophysical properties, thawing rate, algae

Research Paper 31 December 2023

Antimetabolite Prodrug Delivery for Non-small Cell Lung Cancer

Ruda Lee, Sho Tanigawa, Yong Il Park et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 236-243
Mitochondria play an essential role in cancer initiation and progression. Research in the past decades suggested a close relation between mitochondrial dysfunction and cancer, including non-small cell lung cancer (NSCLC). In addition, mitochondrial dysfunction causes excess levels of reactive oxygen species (ROS) generation and induces mitophagy for removing damaged mitochondrial. Therefore, the induction of mitophagy against abnormal mitochondria in cancer cells is expected to be a therapeutic ...
Key Words: mitochondria, redox, prodrug, lung cancer, antimetabolite

December 31, 2023Current Issue Vol. 38 No. 4

    December, 2023 | Volume 38, No. 4
  • Review Paper 2023-12-31

    Recent Advances in Research on Nanotechnology-based Drug Delivery System

    Kyong Min Kwak, Jeong Un Kim, and Moon Il Kim

    Abstract : The term “drug delivery system (DDS)” refers to a group of technologies for efficiently managing the distribution of pharmacologically active medicines to target cells and tissues in vivo. The use of appropriate DDS can enhance the activity, stability, and permeability of pharmaceuticals, as well as their side effects can be reduced by treating only the targeted location and keeping the drug levels below the dangerous threshold in physiological fluids. In particular, materials derived from nanotechnology have received a considerable attention in DDS due to their distinctive advantages, including high loading capacity, effective targeting by the large surface area and abundant surface moieties, unique control of the drug release via a variety of physicochemical stimuli (pH, ionic concentration, heat, light, and chemicals), and efficient delivery of the drug via material-induced penetration through cell or skin membranes. In this review, recent studies on nanotechnology-based DDS, including the benefits of using nanostructured materials in DDS, representative recent nanostructures like lipid nanoparticles and exosomes, as well as DDS that uses nanoparticles to perform multiple functions and nano (micro)needles to deliver the drugs transdermally. Current challenges and prospects for the utilization of nanotechnology in DDS are also discussed.

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  • Research Paper 2023-12-31

    Production of Deinoxanthin Using Deinococcus radiodurans R1

    Young Woo Shin, Sun-Wook Jeong, Younghun Cho et al.

    Abstract : Xanthophyll carotenoids are pigments composed of terpene structures found in various organisms. They have attracted significant attention in the food, nutrition, pharmaceutical, and cosmetic industries due to their remarkable anti-bacterial, anti-inflammatory, and antioxidant activities. Among these, Deinoxanthin, produced by microorganisms of the Deinococcus genus, is known for its superior radical scavenging activity compared to well-known carotenoids like lutein, beta-carotene, and zeaxanthin, owing to its unique structural specificity. The aim of this study was to optimize carbon sources to establish efficient production platform for deinoxanthin using Deinococcus radiodurans. Glycerol proved to be the most effective carbon source for the synthesis of deinoxanthin among those that were examined. From optical density at 600 nm of 37.5 of D. radiodurans, more than 19.8 mg/L of deinoxanthin was produced. Furthermore, the viability of employing inexpensive carbon sources was tested using crude glycerol. A productivity of 20.2 mg/L of deinoxanthin was produced from the optical density at 600 nm of 37.5 of the cell after 72 hours of fermentation. These findings indicated that the D. radiodurans strain might be used to produce carotenoids in a cost-effective manner using cheap carbon source such as crude glycerol and be further employed in a wide range of biotechnological applications, such as food, nutrition, cosmetics, and pharmaceuticals.

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  • Research Paper 2023-12-31

    Effect of Different Light Sources and Intensities on the Growth of Astaxanthin-producing Microalgae Haematococcus lacustris (Chlorophyceae)

    Tae Yeon Yin, Eun Young Yoon, and Tae Hoon Kim

    Abstract : The green microalga Haematococcus lacustris is a source of astaxanthin, a natural pigment additive with commercial value in functional food, cosmetic, and pharmaceutical industries. Although conventional light sources are being increasingly replaced with light-emitting diodes (LEDs), their applications in microalgal biotechnology remain limited. In this study, we investigated the effects of monochromatic light types, light intensities, and different ratios of mixed light on the growth of two H. lacustris strains, UTEX 2505 and UTEX 16. The maximum cell concentrations of UTEX 2505 (944,000 cells mL−1) and UTEX 16 (653,000 cells mL−1) were obtained under blue LED at a light intensity of 100 μmol m−2 s−1. The concentrations of UTEX 2505 and UTEX 16 cells decreased by 43.7% and 53.3%, respectively, under red LED compared with those under blue LED of the same intensity. In monochromatic light experiments, cell concentrations under different light sources decreased in the following order: blue LED > green LED > fluorescent light > red LED. Growth under monochromatic light yielded a higher cell concentration than that under mixed light ratios. Blue LED was the most effective light source for the growth of H. lacustris. The growth of UTEX 2505 and UTEX 16 was the highest under red:blue LED mixing ratios of 2 : 1 and 1 : 1, respectively. In this study, we optimized the light source and intensity for cultivating H. lacustris, providing basic data for further studies on the complex and condition-dependent photosynthetic reactions of H. lacustris, as well as for mass-culture of microalgae for commercial applications.

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  • Research Paper 2023-12-31

    Thermodynamic Properties in the Process of Thawing for Cryopreservation of Algae

    Min Yong Kim, Young Woo Shin, Young Bok Lee et al.

    Abstract : To achieve a high viability by minimizing thawing damage, ice crystallization must be reduced in the process of thawing. Thermophysical properties of suspensions of Porphyra seriata, Undaria pinnatifida, and Sargassum fulvellum were measured to determine the optimal thawing conditions in the thawing process for species conservation of sea algae. The range of final thawing temperature was –2.49 ~ –0.50°C at thawing speed 1.25°C/min and 2~3°C at 1.5~2.0°C/min, and thawing was completed at temperatures above 3.0°C, enabling the removal process of cryoprotectant. The optimum thawing speed with the minimum thawing time was 0.75~1.0°C/min, and the latent heat of thawing was Undaria pinnatifida 128.37 J/g, Porphyra seriata 118.53 J/g, and Sargassum fulvellum 94.42 J/g, which can be useful for the design and manufacture of algae cryopreservation system. From the change of enthalpy, entropy and free energy according to each thawing rate, the optimum thawing rate for recrystallization temperature pan and viability improvement is suitable over Porphyra seriata, Sargassum fulvellum, Undaria pinnatifida 0.75°C/min, 1.0°C/min, 1.5°C/min respectively. From the results of the thawing process, the optimal method of freezing-thawing process of seedlings for aquaculture will be set up to increase the viability and contribute to the aquaculture industry by being used for growth in the farm. In addition, the results of this study will contribute to the establishment of gene bank, seed bank, and seed vault for various kinds of sea algae.

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  • Research Paper 2023-12-31

    Antimetabolite Prodrug Delivery for Non-small Cell Lung Cancer

    Ruda Lee, Sho Tanigawa, Yong Il Park et al.

    Abstract : Mitochondria play an essential role in cancer initiation and progression. Research in the past decades suggested a close relation between mitochondrial dysfunction and cancer, including non-small cell lung cancer (NSCLC). In addition, mitochondrial dysfunction causes excess levels of reactive oxygen species (ROS) generation and induces mitophagy for removing damaged mitochondrial. Therefore, the induction of mitophagy against abnormal mitochondria in cancer cells is expected to be a therapeutic strategy for cancer. In this study, we aimed to develop a mitochondria-targeted delivery system for inducing mitophagy-mediated apoptosis in non-small cell lung cancer (NSCLC). We successfully synthesized amphiphilic glycol chitosan conjugated with a disulfide linker (aGC-SS) and modified paclitaxel with triphenylphosphonium (PTX-TPP prodrug). These components were used to fabricate redox-sensitive nanoparticles loaded with PTX-TPP (aGC-SS-proPTX NPs). The aGC-SS-proPTX NPs exhibited controlled drug release triggered by high glutathione (GSH) levels and showed enhanced accumulation within the mitochondria due to the triphenylphosphonium modification. In vitro experiments demonstrated that the aGC-SH-proPTX NPs effectively suppressed ATP levels, induced mitophagy, and promoted apoptosis in NSCLC cells.Our findings suggest that the targeted delivery of aGC-SS-proPTX NPs triggers mitophagy-mediated apoptosis. This research contributes to the growing understanding of the role of mitochondria in cancer therapy and highlights the potential of targeted mitophagy modulation as a promising approach in NSCLC treatment.

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December 2023 Volume 38, No.4

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Korean Society for Biotechnology and Bioengineering Journal
pISSN 1225-7117 eISSN 2288-8268