Korean Society for Biotechnology and Bioengineering Journal Korean Society for Biotechnology and Bioengineering Journal

pISSN 1225-7117 eISSN 2288-8268

Review Paper 30 September 2023

Recent Research Trends in Mushroom Mycelium-based Materials

Ho-Seong Im, Gyung-Sik You, Yong-Hyeon Jung et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 153-161
Due to global warming and sustainability issues, greenhouse gas reduction and carbon neutralization are essential goals worldwide. As a valuable means of solving environmental problems, mushrooms, and their mycelia are getting much interest from the R&D sectors to the public. In this review, the recent literature on the application of mushroom mycelium as various industrial materials such as leather, building materials, electronic materials, fiber, packaging (alternative plastic), and insulation...
Key Words: mushroom, mycelium, alternative plastics, green battery, biocomposite, mushroom leather

Research Paper 30 September 2023

Optimization of Enzymatic Saccharification with Pretreated Barley Straw of High Loading by Response Surface Methodology

Soo-Yeon Kim, Ji-Bong Choi, Kwang-Soo Kim et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 162-168
In this study, the parameters of the enzymatic hydrolysis process were optimized using response surface methodology to increase the saccharification efficiency with pretreated barley straw of high loading. A central composite design was adopted as the experimental design to investigate the interaction between variables at different levels and derive an optimal point by the fitted model. Through ANOVA analysis, the fitted model was turned to be significant statistically and suitable for explainin...
Key Words: enzymatic hydrolysis, response surface methodology, barley straw, succinic acid.

Research Paper 30 September 2023

Effect of Gas Bubble Characteristics on Fractional Precipitation of (+)-dihydromyricetin

Hyunji Oh and Jin-Hyun Kim Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 169-176
There is an urgent need to elucidate how the introduction of cavitation bubbles or gas bubbles during precipitation improves the precipitation efficiency. In this study, the role of the bubble surface according to the gas flow rate and air sparger pore size in the gas bubble-based fractionation of (+)-dihydromyricetin, a natural bioactive compound, was investigated. As the gas flow rate increased and the air sparger pore size decreased, the bubble surface increased. At gas flow rates of 0.5 to 1...
Key Words: (+)-Dihydromyricetin, gas bubble-assisted fractional precipitation, air sparger pore size, gas flow rate, bubble surface, kinetics

Research Paper 30 September 2023

Antioxidant and Anti-obesity Activities of Roasted Camellia japonica Leaves Extract

Yeo-Jin Lee, Seung-Hwa Yang, Moon-Hee Choi et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 177-187
When natural substances containing polyphenols are heated, the structure of polyphenols have been changed to result in the change of biological activities. In addition, this process imparts new flavors and tastes by preserving moisture and reducing unnecessary flavors. Camellia japonica has been reported to possess physiological activities in various parts of the plant, including leaves (CJL), flower buds, and stems. However, there is a shortage of reports on the physiological activities of extr...
Key Words: Camellia japonica L., polyphenol, antioxidant activity, anti-obesity, HPLC-MS/MS analysis, natural cosmetics

Research Paper 30 September 2023

Optimization of Recombinant Glucagon-like Peptide-1 Production Using Recombinant E. coli

You-Chan Jeon, Deok-Ho Kwon, Yoojin Lee et al. Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 188-193
Obesity is well known as a metabolic abnormality and potentially life-threatening condition associated with several chronic diseases. Various peptide drugs have been developed to treat obesity. In this study, optimization of expression conditions were performed to produce glucagon-like peptide-1 (GLP-1), which is used as an obesity treatment in recombinant E. coli. After the construction of expression vector system and optimization of expression conditions, the productivity of GLP-1 was increase...
Key Words: Glucagon-like peptide-1, Escherichia coli, bioreactor, lactose

Research Paper 30 September 2023

Enhancing Biodiesel Production from Microalgae and Sewage Sludge Lipids by Adding Waste Coffee Ground Lipids

Young Wook Go and Sung Ho Yeom Korean Society for Biotechnology and Bioengineering Journal 2023; 38: 194-202
The free fatty acid (FFA) content in the feedstock lipids of microalgae (MA), sewage sludge (SS), and waste coffee grounds (WCGs) were 7.6%, 38.5%, and 1.7%, respectively. For a single feedstock lipid, the maximum biodiesel conversion was about 70% for MA lipids using acid or alkaline catalysts, 29.6% for SS lipids using acid catalysts, and 93.5% for WCG lipids using alkaline catalysts. For a mixture of MA and SS lipids, the maximum biodiesel conversion was only 38.6% in the experimental range t...
Key Words: biodiesel, microalgae, sewage sludge, waste coffee grounds, lipid mixture, mixing ratio, catalyst type.

September 30, 2023Current Issue Vol. 38 No. 3

    September, 2023 | Volume 38, No. 3
  • Review Paper 2023-09-30

    Recent Research Trends in Mushroom Mycelium-based Materials

    Ho-Seong Im, Gyung-Sik You, Yong-Hyeon Jung et al.

    Abstract : Due to global warming and sustainability issues, greenhouse gas reduction and carbon neutralization are essential goals worldwide. As a valuable means of solving environmental problems, mushrooms, and their mycelia are getting much interest from the R&D sectors to the public. In this review, the recent literature on the application of mushroom mycelium as various industrial materials such as leather, building materials, electronic materials, fiber, packaging (alternative plastic), and insulation has been summarized and discussed. Mushroom mycelium can utilize agricultural and forestry by-products, can be biodegradable, and can control physical properties according to the processing method, so it has highly valuable. In addition, relatively little energy is used, effectively reducing carbon. As a result, mushroom mycelium research should be further developed to create an eco-friendly and healthy future society.

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  • Research Paper 2023-09-30

    Optimization of Enzymatic Saccharification with Pretreated Barley Straw of High Loading by Response Surface Methodology

    Soo-Yeon Kim, Ji-Bong Choi, Kwang-Soo Kim et al.

    Abstract : In this study, the parameters of the enzymatic hydrolysis process were optimized using response surface methodology to increase the saccharification efficiency with pretreated barley straw of high loading. A central composite design was adopted as the experimental design to investigate the interaction between variables at different levels and derive an optimal point by the fitted model. Through ANOVA analysis, the fitted model was turned to be significant statistically and suitable for explaining the data with Adj. R2 value (0.92). The optimal point was suggested as barley straw 20.53%, enzyme 70 mg/g-glucan, incubation time 72 h, saccharification yield was 93.25% with satisfying the 95% prediction interval. Using the optimal point, separate hydrolysis and fermentation was performed to investigate the saccharification reproducibility at scale-up and suitability of saccharification solution for the microbial fermentation.

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  • Research Paper 2023-09-30

    Effect of Gas Bubble Characteristics on Fractional Precipitation of (+)-dihydromyricetin

    Hyunji Oh and Jin-Hyun Kim

    Abstract : There is an urgent need to elucidate how the introduction of cavitation bubbles or gas bubbles during precipitation improves the precipitation efficiency. In this study, the role of the bubble surface according to the gas flow rate and air sparger pore size in the gas bubble-based fractionation of (+)-dihydromyricetin, a natural bioactive compound, was investigated. As the gas flow rate increased and the air sparger pore size decreased, the bubble surface increased. At gas flow rates of 0.5 to 1.5 L/min, the yield and rate constant increased to be 1.7 to 1.9 and 2.6 to 5.5 times (air sparger pore size 10 μm), 1.4 to 1.8 and 1.9 to 4.7 times (30 μm), and 1.3 to 1.8 and 1.4 to 4.2 times (43 μm) higher than those obtained in the conventional precipitation. In addition, the changes in activation energy decreased as the bubble surface increased. The mechanism, by which nucleation could be induced seems to be heterogeneous due to the bubble surface provided.

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  • Research Paper 2023-09-30

    Antioxidant and Anti-obesity Activities of Roasted Camellia japonica Leaves Extract

    Yeo-Jin Lee, Seung-Hwa Yang, Moon-Hee Choi et al.

    Abstract : When natural substances containing polyphenols are heated, the structure of polyphenols have been changed to result in the change of biological activities. In addition, this process imparts new flavors and tastes by preserving moisture and reducing unnecessary flavors. Camellia japonica has been reported to possess physiological activities in various parts of the plant, including leaves (CJL), flower buds, and stems. However, there is a shortage of reports on the physiological activities of extracts subjected to heat treatment. This study evaluated the antioxidant activities, the total phenolics content, total flavonoid content, and anti-obesity activity (α-glucosidase inhibition activity and lipase inhibition activity) of heat-treated C. japonica leaves (CJLH) extract. HPLC and LC-MS/MS techniques were used to verify any alterations in the active ingredient content. Our findings demonstrated that CJL displayed substantial antioxidant and anti-obesity activity. HPLC analysis revealed that catechin was the highest among the polyphenol components. L-Ascorbic acid was detected at high levels in CJL, CJLH1, and CJLH2, but was presumed to be lost due to thermal decomposition in CJLH3 and CJLH4 (The number means different process). In LC-MS/MS analysis, catechin had the highest content and CJLH3 had the highest polyphenol content among the extracts. Based on the data, CJLH can be useful as natural cosmetics, food materials for flavor enhancement and anti-obesity applications.

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  • Research Paper 2023-09-30

    Optimization of Recombinant Glucagon-like Peptide-1 Production Using Recombinant E. coli

    You-Chan Jeon, Deok-Ho Kwon, Yoojin Lee et al.

    Abstract : Obesity is well known as a metabolic abnormality and potentially life-threatening condition associated with several chronic diseases. Various peptide drugs have been developed to treat obesity. In this study, optimization of expression conditions were performed to produce glucagon-like peptide-1 (GLP-1), which is used as an obesity treatment in recombinant E. coli. After the construction of expression vector system and optimization of expression conditions, the productivity of GLP-1 was increased to 11.18 mg/L·h in flask cultivation. Through fermentation conditions were optimized for bioreactor, the GLP-1 productivity was increased to 16.80 mg/L·h in bioreactor, which was 50.27% improvement as compared to the flask cultivation. When 1.00 mM lactose was used as an inducer instead of IPTG, 17.39% higher expression was shown than that with 0.01 mM IPTG.

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  • Research Paper 2023-09-30

    Enhancing Biodiesel Production from Microalgae and Sewage Sludge Lipids by Adding Waste Coffee Ground Lipids

    Young Wook Go and Sung Ho Yeom

    Abstract : The free fatty acid (FFA) content in the feedstock lipids of microalgae (MA), sewage sludge (SS), and waste coffee grounds (WCGs) were 7.6%, 38.5%, and 1.7%, respectively. For a single feedstock lipid, the maximum biodiesel conversion was about 70% for MA lipids using acid or alkaline catalysts, 29.6% for SS lipids using acid catalysts, and 93.5% for WCG lipids using alkaline catalysts. For a mixture of MA and SS lipids, the maximum biodiesel conversion was only 38.6% in the experimental range tested. In contrast, for mixtures of WCG and MA lipids and WCG and SS lipids, where WCG lipids accounted for three-fourth of the mixtures, the biodiesel conversions using an alkaline catalyst were 89.5% and 70.0%, respectively. When MA, SS, and WCG lipids were mixed in equal proportions, 71.8% biodiesel conversion was obtained with 3.0 wt% of an alkaline catalyst. This study shows that WCG lipids, when used with lipids having high FFA content, are crucial for improving the conversion of lipids into biodiesel. In addition, by manipulating the proportion of WCG lipids in the mixture, we were able to use an alkaline catalyst, which has several advantages over an acid catalyst, to produce biodiesel at a relatively high biodiesel conversion.

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September 2023 Volume 38, No.3

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Korean Society for Biotechnology and Bioengineering Journal
pISSN 1225-7117 eISSN 2288-8268